آنتی ­بادی CD27 (EPR8569)

Name: CD27 Antibody Clone EPR8569

Description and applications: CD27 is a glycosylated, type I transmembrane protein with a molecular mass of 50-55 kDa that belongs to the TNF receptor superfamily (TNFRSF7), of which it is its seventh member. This protein is also known as T cell activation antigen, S152 protein and T14 protein. It is encoded by a gene located in the chromosomal region 12p13.31. As a member of the tumour necrosis factor (TNF) receptor family, CD27 plays an important role in cell growth and differentiation as well as in apoptosis regulation. In the former case, CD27 binds to CD70, its ligand, and plays an important role in costimulation of T cell activation and regulation of B cell differentiation and proliferation and immunoglobulin synthesis. The cytoplasmic domains of CD27 have also been shown to interact with TRAF2 and TRAF5 to promote activation of NF-kappa B and SAPK/JNK. Its role inapoptosis is linked to its ability to bind to the proapoptotic SIVA protein; thus, CD27 plays an important role in inducing this cell death process. CD27 is expressed in medullary thymocytes, virtually all mature T cells, memory B cells, NK cells, and a fraction of normal plasma cells but not in virgin B cells or effector T cells. In patients diagnosed with acute lymphoblastic leukaemia (ALL), overexpression of CD27 is associated with a more favourable prognosis and an increased anti-leukaemic immune response by the organism. Immunohistochemical techniques have confirmed the expression of the CD27 antigen in isolated cases of mantle cell lymphoma, Burkitt lymphoma, marginal zone lymphoma, and plasmacytoma. In cases of multiple myeloma, apparently the expression of CD27 has predictive value for patient survival, with an overall three-year survival rate of 92% in positive cases compared to 50% in negative cases. CD27 negativity in cases of hairy-cell leukaemia diagnosed in the spleen has been suggested as a useful tool in the differential diagnosis with other types of B cell leukaemia.

Composition: Anti-human CD27 mouse monoclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Immunogen:Human recombinant protein similar to CD27.