آنتی­ بادی CD45RB (BRA-11)

Name: CD45RB Antibody Clone BRA-11

Description and applications: CD45 has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45 are distributed through-out the immune system according to cell type. These isoforms arise because of alternative splicing of exons 4, 5 and 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out). The variation in these isoforms is localized to the extracellular domain of CD45, while the intracellular domain is conserved. CD45 functions as a phosphotyrosine phosphatase, a vital component for efficient tyrosine phosphorylation induction by the TCR/CD3 complex. The tyrosine phosphatase activity of CD45 is contained within the conserved intracellular domain. Src and Syk family protein tyrosine kinases are utilized by the TCR/CD3 complex to initiate signaling cascades. Several members of these two families, including Lck, Fyn and ZAP-70, have been implicated as physiological substrates of CD45. CD45RB is expressed in a high proportion of circulating B lymphocytes, suppressor and cytotoxic T lymphocytes, a subpopulation of helper T lymphocytes and in most thymocytes. Expression of CD45RB decreases as the T cells mature from naïve to memory mature T cells, suggesting that its expression is predominantly in immature T lymphocytes. The anti-CD45RB antibody is useful in the study of lymphocyte subpopulations in autoimmune diseases, especially inflammatory bowel disease, and for the immunophenotypic characterization of leukemias and lymphomas.

Composition: Anti-human CD45RB mouse monoclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.