Category: immunohistochemistry

Name: Keratin 13 Monoclonal Antibody Clone EP69

Description and aplications: The nomenclature coined in 1982 by Moll and Franke assigned ranges from 1 to 8 for cytokeratin type II (neutral or basic) and between 9 and 21 for type I (acidic). However and because of the high homology among the different molecules is common for a single monoclonal antibody to react with different types of keratins , for example keratin brand AE1 keratins 10, 14 , 15, 16 and 19. The 54 kD keratin 13 is the major acidic keratin that is assembled in vivo with type 4 and is expressed in the suprabasal cells of the multilayered keratinizing epithelium of mucous membranes, transitional epithelium, pseudostratified epithelium and myoepithelial cells. The monoclonal anti- keratin 13 antibody has been used as a marker for non-keratinized squamous epithelium and is expressed in various squamous metaplasias, but is down regulated in squamous dysplasia and squamous carcinoma. It is useful to identify epithelial derived tumors of the trachea, apocrine and eccrine sweat glands, salivary glands, stem cells of the endocervical glands, bladder, exocervix , tongue, esophagus, anus and basal layer of the epidermis.In addition, mutations in the gene sequence of keratin 13 have been implicated in genetic disease known as autosomal dominant white sponge nevus of the oral cavity.

Composition: anti-Keratin 13 rabbit monoclonal antibody obtained from supernatant culture and prediluted in a tris buffered solution pH 7.4 containing 0.375mM sodium azide solution as bacteriostatic and bactericidal. The amount of the active antibody was not determined.

Immunogen: Synthetic peptide corresponding to human keratin 13 residues.

Name: Prolactin Antibody clone EP193

Description and applications: Prolactin is a peptide hormone secreted by the anterior pituitary that is necessary for the proliferation and differentiation of the mammary glands. Prolactin also acts in a cytokine-like manner and as an important regulator of the immune system. Prolactin has important cell cycle related functions as a growth, differentiating and antiapoptotic factor. Prolactin is secreted by lactotrophs in the anterior pituitary. Prolactin producing cells make up approximately 20 percent of the pituitary. Elevated counts of these cells have been observed in pregnant women, newborns and in multiparous women. An antibody to prolactin is useful for the identification of pituitary tumors.

Composition: Anti-human Prolactin rabbit monoclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide

Immunogen: A synthetic peptide corresponding to residues of human Prolactin protein

Name: PSA antibody Clone ER-PR8

Description and applications: PSA is an antigen present in prostatic tissue and in the majority of prostatic carcinomas. This antibody recognizes primary and metastatic prostatic neoplasms and rarely in tumors of nonprostatic origin. These include breast and a minority of salivary gland tumors. The antigen is a 33-34 kD glycoprotein that is restricted to epithelial cells of the prostate. Because of the high specificity of PSA for prostate glandular epithelium, the antibody is useful to identify prostatic carcinomas of the prostate and their frequent infiltration of the surrounding organs (eg .: rectum and urinary bladder). Rectal adenocarcinomas, urothelial carcinoma or adenocarcinoma of the urinary bladder did not express PSA. The loss of immunoreactivity for PSA which can occur in poorly differentiated neoplasms cannot completely exclude the diagnosis of prostate cancer with a negative PSA. Another important use is for differential diagnosis and determine the origin of the primary tumor with metastases of unknown origin, especially lymph node and bone. PSA can be used together with the Prostein, which has at least the same sensitivity and is slightly more specific tha PSA.

Composition: anti-human PSA mouse monoclonal antibody purified from supernatant diluted in tris bffered saline, pH 7.3-7.7, with protein base, and preserved with sodium azide.

Name: IgG Antibody Clone E20-V

Description and applications: The antibody labels the IgGs contained in the plasma cells and their precursors. In general, membrane bound immunoglobulins, extracellular immunoglobulins bound to connective tissue or blood vessels, and immunocomplexes can only be demonstrated in frozen tissues. Plasma cells may not
be intensely stained in frozen tissue since the immunoglobulins are distributed diffusely through the cytoplasm of these cells. This antibody is useful for identifying leukemias, plasmacytomas and B cells derived from Hodgkin lymphomas. Due to the restrictions on the expression of light and heavy chains in these diseases, demonstration of B-cell lymphomas is possible with clonal rearangements studies. Another application is

Composition: Anti-human IgG rabbit monoclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Name: IgG4 Antibody Clone EP138

Description and applications: Human IgG4, one of four subclasses of IgG, contains a gamma 4 heavy chain and a hinge region that is shorter than that of IgG1. No allotypes have been detected on the heavy chains of IgG4. Its two primary effector functions are activating complements and binding to the FcgR of effector cells to initiate phagocytosis. Human IgG4 accounts for less than 6% of the total IgG serum level. Recent studies show that serum levels and immunohistochemistry staining with IgG4 antibody is a useful diagnosis marker for IgG4-related sclerosing diseases. A new concept of IgG4-related systemic disease (ISD) has been established recently. The ISD is characterized by elevated serum IgG4 levels and extensive IgG4+ plasma cell infiltrate in pancreas and/or in other organs, including peripancreatic tissue, bile duct, gallbladder, portal area of the liver, gastric mucosa, colonic mucosa, salivary glands, lymph nodes, and bone marrow. Immunohistochemistry analysis of IgG4 is useful for identifying ISD.

Composition: anti-human IgG4 rabbit monoclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Name: Inhibin Alpha Antibody (Clone R1)

Description and applications: This antibody recognizes the 32kDa alpha subunit of human inhibin. Inhibin alpha is expressed in a range of tissues including prostate, brain, adrenal gland, testis and ovary. The antibody may be of value in the differentiation of adrenocortical tumors, placental and gestational trophoblastic lesions and sex cord stromal tumors.

Composition: anti-human Inhibin alpha mouse monoclonal antibody purified from ascites. Prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Name: Insulin Antibody Clone EP125

Description and applications: Insulin is a hormone that regulates glucose homeostasis. It increases cell permeability to monosaccharides, amino acids and fatty acids, and it accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. It is synthesized in the beta cell of the pancreas. The antibody labels both normal and neoplastic insulinproducing cells. It is useful in identifying insulinoma.

Composition: Anti-human Insulin rabbit monoclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Name: Involucrin Antibody Clone SY5

Description and applications: Involucrin is expressed in a range of stratified squamous epithelia, including the cornea. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures it is expressed by all cells that have left the basal layer. Involucrin expression is abnormal in squamous cell carcinomas and premalignant lesions, and is reduced in severe dysplasias of the larynx and cervix.

Composition: Anti-human Involucrin mouse monoclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Name: Keratin 18 Antibody Clone DC-10

Description and applications: The nomenclature coined in 1982 by Moll and Franke assigned ranges from 1 to 8 for keratin type II (neutral or basic) and between 9 and 21 for type I (acidic). However and because of the high homology among the different molecules is common for a single monoclonal antibody to react with different types of keratins , for example keratin brand AE1 keratins 10, 14 , 15, 16 and 19. This antibody reacts with the acid intermediate filament protein of 45 kDa keratin, identified as Keratin 18. Keratin 18 is generally co-expressed with the keratin 8 in most simple gland epithelia. In general, the keratin 18 does not react with squamous multilayered epithelium. In neoplastic tissues, this antibody reacts with different benign and malignant epithelial lesions. Most adenocarcinomas and basal cell carcinomas express this keratin, whereas it is absent in squamous cell carcinomas.

Composition: anti-Keratin 18 mouse monoclonal antibody obtained from supernatant culture and prediluted in a tris buffered solution pH 7.4 containing 0.375mM sodium azide solution as bacteriostatic and bactericidal. The amount of the active antibody in the sample is 3,3 μg/mL..

Immunogen: Human breast cancer PMC 42 cells.

Name: LIN-28 Antibody Clone EP150

Description and applications: LIN28 is a highly conserved, RNA-binding protein (RBP). It plays an important role as a translational enhancer, leading specific mRNAs to polysomes and therefore increasing the competence of protein synthesis. LIN28 was identified as a negative regulator of miRNA biogenesis and suggested to play a central role in blocking miRNAmediated differentiation in stem cells and certain cancers. LIN28 is expressed by various undifferentiated embryonic cell types. Anti- LIN28 has been used as a sensitive marker for germ cell tumors. The positive staining of LIN28 in yolk sac tumors showed an advantage over OCT4, which is negative in these tumors.

Composition: Anti-human LIN-28 rabbit monoclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.