Category: monoclonal

Name: Rabbit Anti-Human PRAME 1 Monoclonal Antibody (Clone EPR20330)

Description and aplications: The PReferentially expressed Antigen in MElanoma (PRAME), or LB33-E antigen, is part of the family of melanoma nuclear proteins recognized by cytolytic T lymphocytes and is encoded by a gene of the same
name (PRAME) located on chromosomal region 22q11.22. The PRAME protein has been characterized as a dominant inhibitor of the retinoic acid receptor, thus participating in the blockade of cell proliferation, differentiation or apoptosis induced by retinoic acid through the RARA, RARB and RARG receptors. Thus, overexpression of PRAME in tumor cells confers a survival advantage over normal cells. In melanomas, PRAME inhibition restores retinoic acid signaling an reinstates the sensitivity of tumor cells to the antiproliferative effects of this molecule.

Composition: Anti-human PRAME 1 rabbit monoclonal antibody obtained from ascitic and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Immunogen: N/A

Name: Mouse anti- Human Renal cell carcinoma marker (PN-15) Monoclonal Antibody

Description and aplications:This antibody detects the membrane glycoprotein gp200. In a normal kidney, gp200 is located along the brush border of the contoured and straight segments of the proximal tubule, as well as focally along the luminal surface of Bowman’s capsule, contiguous with the exit of the proximal tubule. Gp200 is also located on the luminal surface of the mammary ducts and lobes and the tubular epithelium of the epididymis, in the cytoplasm of parathyroid and parenchymal cells, and focally within the colloid of thyroid follicles.

Composition: Anti-human renal cell carcinoma marker (PN-15) mouse monoclonal antibody purified and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Immunogen: Microsomal fraction of human renal cortical tissue homogenate.

Name: Mouse Anti-Human SMAD4/Dpc4 Monoclonal Antibody (Clone B8)

Description and applications: The Mothers Against Decapentaplegic homolog 4(SMAD4) protein, also known as MADH4, MANrelatedprotein 4, MAD, pancreatic carcinoma deletion factor 4 or DPC4, is linked to the SMAD4 suppressor gene, which consists of 11 exons, and is located in the genomic region 18q21.1. This gene shows allelic or biallelic loss in 50% of pancreatic carcinomas

Composition: Anti-human SMAD4/Dpc4 mouse monoclonal antibody obtained from ascitic fluid and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide

Immunogen: N/A

Specification:

C-erbB2 (also called Her-2/neu, ERBB2 or neu) is a transmembrane receptor tyrosine kinase. HER-2 is considered as the target oncogene driving the amplification, so that its activation will causes malignant transformation and increases the malignant potential (cell proliferation, invasiveness etc.) of the cells. Amplification of C-erbB2 gene invariably leads to overexpression of its protein product. Over-expressed C-erbB protein disturbs the HER-receptor family signaling networks, i.e. signaling mediated via EGFR receptor.

Presentation:

Supernatant contains 15mM sodium Azide

Specification:

The PAX-5 gene is essential for B-cell differentiation. There are at least four isoforms, of which PAX-5a has been most studied. PAX-5 encodes the 50 kDa B-cell specific activator protein, BSAP. PAX-5 is expressed by pro-, pre-and mature Bcells, but is downregulated during terminal differentiation of plasma cells. PAX-5 influences the expression of other B-cell specific genes, including CD19 and CD20 and CD79a, preceding the expression of CD20. PAX-5 is silenced at the plasma cell stage under the influence of B-lymphocyte-induced maturation protein-1 (PRDM1). PAX-5 is expressed during mouse embryogenesis within the developing brain in a way that is temporarily and spatially tightly condoled. PAX-5 deficient mice show deformation of the mid-brain. Expression in human embryogenesis occurs in the mesoencephalon and spinal cord.

Presentation:

Tissue culture supernatant containing 15mM sodium azide

Specification:

The Ki-67 protein is a nuclear protein doublet, 345-395 kDa, playing a pivotal role in maintaining cell proliferation. In diagnostic histopathology and cell biology, the antibody has proven valuable for the demonstration of the Ki-67 antigen in normal and neoplastic cells, for example in soft-tissue sarcoma, prostatic adenocarcinoma, and breast carcinoma. The Ki-67 has been confirmed as a very powerful single prognostic factor for overall survival, with highly proliferative cases showing a much poorer outcome than tumors with low proliferation. In breast cancer, the proliferative index measured by Ki-67 immunoreactivity has both prognostic and predictive value.

Presentation:

1ml Ki-67 tissue culture supernatant contains 15mM Azide

Specification:

Progesterone Receptor is a protein with 946 amino acids, is a ligand-activated transcription factor member of the steroid receptor super family of nuclear receptors. Progesterone Receptor is predominantly expressed in female sex steroid responsive tissues such as the mammary gland, uterus and ovary. But Progesterone Receptor is also found in other tissues such as endocrine cells of the Langerhans islets. Progesterone Receptor exists in two isoforms, Progesterone Receptor alpha and Progesterone Receptor-β, transcribed from two promoters by a single gene. The two Progesterone Receptor isoforms are identical except that Progesterone Receptor-alpha lacks 164 amino acids contained at the N-terminal end of the Progesterone Receptor-β. Progesterone Receptor alpha is the major isoform in the uterine stroma, and Progesterone Receptor-β is the predominant isoform in the endometrial glands.

Presentation:

Supernatant contain 15mM Sodium Azide

Specification:

Chromogranin A is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It is an excellent marker for carcinoid tumors, pheochromocytomas, paragangliomas, and other neuroendocrine tumors. Co-expression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms. Reportedly, co-expression of certain keratins and chromogranin indicates neuroendocrine lineage. The presence of strong anti-chromogranin staining and absence of anti-keratin staining should raise the possibility of paraganglioma. The co-expression of chromogranin and NSE is typical of neuroendocrine neoplasms. Most pituitary adenomas and prolactinomas readily express chromogranin.

Immunogen:

Human pheochromocytoma

Presentation:

Bioreactor Concentrate with 0.05% Azide

Specification:

This MAb reacts with an extracellular domain (close to transmembrane) of CD56/NCAM. Three isoforms of neural cell adhesion molecule (NCAM) are produced by differential splicing of the RNA transcript from a single gene. The 135kDa isoform is the basic molecule, which is glycosylated or sialylated to produce the mature species. CD56/NCAM is reported to express on most neuroectodermal derived cell lines, tissues, and neoplasms such as retinoblastoma, medullblastoma, astrocytoma, and neuroblastoma. It is also expressed on some mesodermally derived tumors such as rhabdomyosarcoma and also on natural killer cells.

Immunogen:

Membrane preparation of a small cell lung carcinoma

Presentation:

Bioreactor Concentrate with 0.05% Azide