Name: Adipophilin Antibody (Polyclonal)
Description and applications: Sebaceous carcinoma is a relatively uncommon cutaneous malignancy and mimics other malignant neoplasms, such as basal and squamous cell carcinomas, and benign processes, such as chalazions and blepharitis, sometimes resulting in delayed diagnosis and suboptimal treatment. Adipophilin is present in milk fat globule membranes and on the surface of lipid droplets in various normal cell types. Recently, it has been reported that adipophilin was expressed in sebaceous adenomas with a specific pattern: membranous with strong uptake at the periphery of intracytoplasmic lipid vacuoles. Sebaceous carcinomas are also labeled with a similar pattern. Additionally, in cases of poorly differentiated sebaceous carcinoma, adipophilin highlights the sebocytes with a strong membranous labeling of intracytoplasmic lipid droplets. Moreover, xanthelasmas, xanthogranulomas, xanthomas, metastatic renal cell carcinomas were also weakly-to- moderately positive for adipophilin in one study. Expression of adipophilin with a membranous pattern of staining was not seen in any of the other clear cell lesions of the skin, including basal and squamous cell
carcinomas, trichilemmomas, clear cell hidradenomas,or balloon cell nevi. Interestingly, a nonspecific granular uptake of anti-adipophilin was seen in adjacent macrophages, keratohyalin granules of epithelial squamous cells, and some tumor cells.Therefore, this anti-adipophilin is suitable for immunostaining formalin-fixed, paraffin-embedded tissue and is helpful in the identification of intracytoplasmic lipids, as seen in sebaceous lesions. Acinary cell carcinomas of the breast, some liposarcomas, or carcinomas of the colon, lung, pancreas or prostate and strangely the majority of Burkitt lymphomas might also be positive.
Composition:Anti-human Adipophilin rabbit polyclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.