آنتی­ بادی ARG-1 (EP261)

Name: Arginase-1 Antibody (Clone EP261)

Description and applications: Arginase is a manganese metalloenzyme that catalyzes the hydrolysis of arginine to generate ornithine and urea. Arginiase I and II are isoenzymes which differ in subcellular localization, regulation, and possibly function. Arginase I is a cytosolic enzyme, which is expressed mainly in the liver as part of the urea cycle, whereas arginase II is a mitochondrial protein found in a variety of tissues. Antibody to ARG-1 labels hepatocytes in normal tissues and granulocytes in peripheral blood. ARG-1 is a sensitive and specific marker for identification of hepatocellular carcinoma. This antibody is very useful in distinguishing between: 1) liver metastases of various adenocarcinomas and hepatocellular carcinoma (HCC), which can be a realdiagnostic challenge, especially in small biopsies or material from fine needle aspiration ( FNA) and 2) the distinction between different histological variants of HCC and cholangiocarcinoma. Specifically, the ARG-1 antibody is key in the diagnosis of scirrhous hepatocellular carcinoma, where specific markers for adenocarcinomas are generally positive while HepPar-1 in some cases it may be negative. In addition, several studies have shown that ARG-1 antibody is more sensitive than HepPar-1 for immunohistochemical diagnosis of hepatocellular carcinoma while the second is usually negative in poorly differentiated hepatocellular carcinomas and can be positive in adenocarcinomas of pancreatic, gastric, colic origin or even cholangiocarcinoma. However, isolated cases of pancreatic adenocarcinomas and cholangiocarcinomas presented focal staining against ARG-1. In this line, it should be considered that the ARG-1 antibody does not allow differential diagnosis between benign, dysplastic and malignant hepatocyte lesions.

Composition: anti-human ARG-1 rabbit monoclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.