Name: Rabbit anti-human BOB-1 Polyclonal Antibody
Description and applications: The BOB.1, OBF.1, OCA-B (now called BOB.1 / OBF.1) is a 35 kD protein encoded by a gene located on chromosome 11q23.1. BOB.1 from “B cell binding Octamer protein 1 “is a specific coactivator of transcription factors Oct-1 and Oct-2 of B lymphocytes. BOB.1 mission is to increase transcriptional activity of such proteins. Interaction with transcription factor OCT2 octamer ATGCAAAT nucleotides is critical and decisive for the expression of immunoglobulin genes. It has been recently demonstrated that alteration in immunoglobulin transcription is correlated with the downregulation of the transcription factors Oct-2 and BOB.1 / OBF.1 on B lymphocytes. This antibody recognizes BOB.1 of human, murine and rat origin. In normal lymphoid tissue, BOB.1 is expressed mainly in the nuclei of germinal center B lymphocytes, with lower intensity in the follicular mantle and dispersed form in B lymphocytes localized in the interfolicular areas of the lymphoid tissue; for this reason it is useful in identifying B cell lymphomas. Variable intensity has been demonstrated in Hodgkin disease, classic type without statistical relationship with its different subtypes. The expression of specific activator proteins of B lymphocytes like “octamer-binding transcription factor 2” [Oct-2] and BOB.1 with pan-B lymphocyte markers (CD20 or CD79a ) are useful for the differential diagnosis of diffuse large B-cell lymphoma, nodular lymphocyte-predominant Hodgkin lymphoma (OCT-2 + / BOB.1 + / PAN-B +) with anaplastic lymphoma B (OCT-2 / BOB. 1- / PAN-B +). The antibody shows no cross-react with Oct-1 or Oct-2.
Composition: anti-human BOB-1 rabbit monoclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide
Intended use: Immunohistochemistry (IHC) on paraffin embedded tissues. Not tested on frozen tissues or Western-Blotting