آنتی ­بادی CD33 (PWS44)

Name: CD33 Monoclonal Antibody (Clone PWS44)

Description and applications: Clone PWS44 detects the CD33 antigen on the membrane and in the cytoplasm of cells of myelomonocytic lineages. In normal bone marrow trephine biopsies, clone PWS44 stains myeloid and myelomonocytic hemopoiesis and mature macrophages; cells of the erythroid and megakaryocytic series are negative. Some reactivity was observed in cytotrophoblasts and syncytiotrophoblasts of placenta. Except for dendritic cells and infiltrating macrophages, no staining was observed in other normal tissues evaluated. Clone PWS44 stained acute myeloid leukemias, chronic myeloid leukemia, granulocytic sarcomas and cases of myeloid dysplasia and myeloproliferative diseases. PWS44 stained cases of non-lymphoid tumours. In the lymphoid neoplasms tested CD33 staining was observed in precursor B-lymphoblastic leukemias, precursor T-lymphoblastic leukemias, classical Hodgkin’s lymphomas, Burkitt lymphomas, plasma cell neoplasms, hematodermic lymphoid neoplasms, anaplastic large cell lymphoma, lymphoplasmacytic lymphoma.

Composition: anti-human CD33 mouse monoclonal antibody purified from tissue culture supernatant. Prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Immunogen: Prokaryotic recombinant protein corresponding to a region of the C2 domain of the human CD33 molecule.