آنتی­ بادی Uroplakin III (BC17)

.(Name: Uroplakin III Antibody (Clone BC17

Description and aplications: This antibody detects the Uroplakin III, a glycoprotein of 47 kDa that forms the asymmetric part of the membrane plates unit constituting the apical surface of the urothelial cell surface or “umbrella cells”. In non-neoplastic cells the uroplakin III is detected exclusively in the normal urothelium of the bladder, ureter and renal pelvis. The antibody stains the luminal surface of the plasma membrane of the “umbrella cells” and weakly in the cytoplasm of some of these cells and some deeper isolated urothelial cells. In tumors, Uroplakin III is detected in 60% of primary urothelial carcinomas and up to 53% of metastases (sensitivity 0.57). Brenner tumors of the ovary also show immunoreactivity for Uroplakin III, the remaining non-urothelial neoplasms being negative (specificity 1.00). Markers such as Uroplakin III, p63, thrombomodulin and GATA3 are expressed in a high percentage of urothelial carcinomas and are usually negative in renal carcinomas.

Composition: anti-Uroplakin III rabbit monoclonal antibody obtained from supernatant culture and prediluted in a tris buffered solution pH 7.4 containing 0.375mM sodium azide solution as bacteriostatic and bactericidal.

.Immunogen: Synthetic peptide derived from the Cterminus of human Uroplakin III