Category: immunohistochemistry

Name:Antibody Serotonin clone 5HT-H209

Description and applications: Serotonin-positive cells are present in gastric, duodenal, jejunal, ileal, colonic, and appendiceal mucosae. Positive cells are sparse and usually occur in the lower half of the gastric and intestinal mucosae. In the appendix, in addition to the mucosal serotonincontaining cells, there are single or small clusters of serotoninpositive cells in the sub-epithelial region of the lamina propria.

Composition: Anti-human Serotonin mouse monoclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide

Name:Antibody Skeletal Muscle Actin clone 5C5,F8,C7

Description and applications: 5C5.F8.C7 MAb is highly specific and shows no crossreaction with smooth muscle actin. This antibody reacts with sarcomeric actins of normal tissues and neoplasms derived from such tissues (i.e. rhabdomyosarcomas).

Composition: Anti-human Skeletal Muscle Actin mouse monoclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide

Name: P62 Antibody clone LCK LIGAND 3/P62

Description and applications: This antibody recognises phosphotyrosine p62, a member of the c-src family of cytoplasmic kinases, which binds as a ligand to the SH2 domains of p56 (Lcr) in the absence of phosphotyrosine. p62/SQSTM1 is an adapter protein targeting the protein aggregates ubiquitinated by lysosomal degradation. p62/SQSTM1 is selectively degraded via the autophagic pathway. Mallory’s hyaline and intracellular hyaline bodies are associated with chronic non-neoplastic liver diseases (steatohepatitis, related or not to alcohol abuse; toxic or metabolic chronic cholestasis) and with neoplastic liver diseases such as hepatocellular carcinoma. These inclusions are related to and contain keratin aggregates, especially keratin 8, ubiquitin, heat shock proteins, and the stress adaptor protein p62, stabilized by bonds catalysed by transglutaminases. P62 binds to ubiquitin and acts as an adaptor to bind ubiquitinated proteins. Autophagy is an evolutionary highly conserved degradative mechanism affecting the components of the cytoplasm that contributes to homoeostasis by making organelle replacement possible. Unlike the ubiquitin-proteosome system, the autophagy mechanism occurs in multiple steps that include the formation of the phagophore, which traps proteins and organelles for its degradation, and the autophagosome, which, through mechanisms involving cytoskeleton’s microtubules, fuses with lysosomes to form autolysosomes, where the material is eventually degraded. This antibody is useful for the identification of p62, found in Mallory’s hyaline and intracellular hyaline bodies present in chronic liver diseases and hepatocellular carcinomas. It is also useful to detect p62 in autophagic vacuoles present in various neurodegenerative, neoplastic infectious, and inflammatory neuromuscular diseases.

Composition: Anti-human P62 mouse monoclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide

Name: Pancreatic lipase Antibody clone EPR6275

Description and applications: Pancreatic lipase is a 56kDa molecular mass enzyme actively secreted in the duodenum by the pancreas in the presence of bile salts and colipase. It belongs to the family of triglyceride lipases, of which there are at least three tissue-specific isoenzymes (pancreatic, hepatic and gastric and lingual) that break down dietary fats by converting triglycerides into monoglycerides and free fatty acids. Pancreatic lipase together with bile salts favors the absorption into the lymphatic system of the monomers resulting from digestion. Human pancreatic lipase is encoded by the PNLIP gene of 13 exons and more than 20 kb, located on the chromosomal region 10q25.3. In normal tissues this antibody identifies pancreatic acinar cells and in neoplasms it recognizes acinary cell carcinoma of the pancreas, which expresses this marker in more than 50% of cases. For this reason and integrated in a panel of suitable antibodies, pancreatic lipase allows the differentiation of acinar adenocarcinoma of pancreas from other malignancies with similar morphology (especially neuroendocrine carcinoma with acinary architecture) and establish the pancreatic origin of distant metastases, that on numerous occasions may be the first manifestation of the tumor.

Composition: Anti-human Pancreatic lipase mouse monoclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide

Name: Cytokeratin 14 antibody clone LL002

Description and aplications: Cytokeratin 14 belongs to the type A (acidic) subfamily of low molecular weight keratins and exists in combination with keratin 5. Cytokeratin 14 has been studied as a prognostic marker in breast cancer. Here it is used as a marker for the myoepithelial cells and for the identification of basaloid histotype of breast carcinoma.
The antibody also distinguishes stratified epithelial cells from simple epithelial cells and has been reported useful in the identification of squamous cell carcinomas.

Composition: anti-human Cytokeratin 14 mouse monoclonal antibody purified from ascites fluid by Protein G chromatography. Prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide

Immunogen: A synthetic peptide of 15 amino acid residues from the C-terminus of human keratin 14.

Name:Antibody Calponin clone EP63

Description and applications: Calponin is a smooth muscle specific, actin-, tropomyosin- and calmodulin-binding protein thought to be involved in regulation of actomyosin as well as the regulation or modulation of contraction. It is expressed on smooth muscle cells and myoepithelial cells. Calponin antibody has been used to identify invasion of breast lesion. Additionally, Calponin is expressed on malignant fibrous histiocytoma of bone and adenoid cystic carcinoma of salivary gland. The consistently positive staining pattern in adenoid cystic carcinomas may be useful in discriminating

Composition: Anti-human Calponin rabbit monoclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide

Name: Delta Catenin-1 (p120)  (Clone EP66)

Description and applications:Catenins are proteins that are linked to the cytoplasmic domain of transmembrane cadherins. p120 Catenin is a member of this Armadillo gene family of junctional plaque proteins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be important for cadherins cell-adhesion properties. Cytoplasmic accumulation of p120 Catenin has been observed in lung cancer, pancreatic cancer, gastric cancer and colon cancers and is associated with poor progress in colon cancer patients. In breast lobular neoplasia, anti p120 Catenin shows a diffuse cytoplasmic immunostaining pattern, while breast ductal neoplasia retains the membrane immunostaining pattern. p120 Catenin antibody is useful in differentiation of lobular carcinoma from ductal carcinoma of the breast and in identifying early lesions of lobular neoplasia.

Composition:Anti-human Delta Catenin-1 (p120) rabbit monoclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Immunogen: A synthetic peptide corresponding to residues in human p120 Catenin protein.

Name: Epithelial Membrane Antigen(EMA)-CloneE29

Description and applications: Anti-EMA antibody is a useful marker for staining many carcinomas. It stains normal and neoplastic cells from various tissues, including mammary epithelium, sweat glands and squamous epithelium. Hepatocellular carcinoma, adrenal carcinoma and embryonal carcinomas are consistently EMA negative, so keratin positivity with negative EMA favours one of these tumours. EMA is frequently positive in meningioma, which can be useful when distinguishing it from other intracranial neoplasms, e.g. Schwannomas. The absence of EMA can also be of value since negative EMA staining is characteristic of some tumours including adrenal carcinoma, seminomas, paraganglioma and hepatoma. Many mesotheliomas, epithelioid and synovial sarcomas, chordomas, choroid plexus tumors, nodular lymphocyte-predominant Hodgkin lymphoma, anaplastic CD30 / ALK positive lymphomas and myelomas are positive; occasionally some small round cell tumours and small cell sarcomas, T null phenotype or CD56 / CD57 + T / NK lymphomas are positive. Other lymphomas, basal cell carcinomas, hepatocellular carcinomas, melanomas, endocrine neoplasms and soft tissue tumours are consistently negative.

Composition: anti-human EMA mouse monoclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Name: Fascin  (Clone FCN01)

Description and applications:Anti-Fascin is a very sensitive marker for ReedSternberg cells and variants in nodular sclerosis, mixed cellularity, and lymphocyte depletion Hodgkin’s disease. It is uniformly negative in lymphoid cells, plasma cells and myeloid cells. Anti-Fascin is positive in dendritic cells. This marker may be helpful in distinguishing between Hodgkin’s disease and nonHodgkin’s lymphoma in difficult cases. Also, the lack of expression of Fascin in the neoplastic follicles in follicular lymphoma can be helpful in distinguishing these lymphomas from reactive follicular hyperplasia in which the number of follicular dendritic cells is normal or increased. Anti-Fascin has been suggested as a prognostic marker in neuroendocrine neoplasms of the lung, as well as ovarian cancer.

Composition: Anti-human Fascin mouse monoclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Name: Rabbit anti-human GH (Growth Hormone) Monoclonal Antibody clone EP267

Description and applications: Growth hormone (GH or hGH), also known as somatotropin or somatropin, is a peptide hormone that is produced and secreted by somatotrophs of the anterior pituitary gland. GH exerts a wide variety of biological actions in many different tissues and cell types. The actions of GH at the cellular level can be divided into three categories: those affecting mitogenesis, differentiation, and metabolism.The GH antibody specifically labels somatotrophs in pituitary in normal tissues. It is useful in classification of pituitary tumor.

Composition: Anti-human GH (Growth Hormone) rabbit monoclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide

Immunogen: A synthetic peptide corresponding to residues of human Growth Hormone protein