آنتی‌ بادی Cytokeratin 8/18/19 (B22.1&B23.1/BA17)

Name: Cytokeratin 8/18/19 Antibody clone B22.1&B23.1/BA17

Description and application: Keratins are cytoplasmic intermediate filament proteins expressed by epithelial cells. Cytokeratins 8 & 18 (CK 8 & 18) can be found in most simple epithelia, e.g. thyroid, female breast, gastrointestinal tract, and respiratory tract. Adenocarcinomas and most non-keratinizing squamous carcinomas will stain with anti-CK 8 & 18, but keratinizing squamous carcinomas will not. This antibody is used when attempting to demonstrate the presence of Paget cells; there is very little keratin 18 in the normal epidermis so this will only stain Paget cells. The use of immunostaining facilitates the interpretation and has been shown to be more sensitive than mucin histochemistry. Cytokeratin 19 is a member of type I acidic subfamily of intermediate filaments. It is expressed in various different human tissues except in liver. Keratin 19 is not expressed in hepatocytes, therefore, antibody to keratin 19 is useful in the identification of liver metastasis.

Composition: Anti-human Cytokeratin 8/18/19 Mouse monoclonal antibody prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Intended use: Immunohistochemistry (IHC) on paraffin embedded tissues. Not tested on frozen tissues or Western-Blotting