آنتی بادی Epidermal Growth Factor Receptor (EP22)

.(Name: EGFR (Epidermal growth factor receptor) Antibody (Clone EP22

Description and applications: The epidermal growth factor receptor (EGFR) is a transmembrane glycoprotein of 170 kDa with tyrosine-kinase activity after binding to the epidermal growth factor (EGF) and is involved in cell growth and differentiation. Tyrosine(Tyr) residues Tyr 1068, Tyr 1148 and Tyr 1173 located at the carboxy (C) terminal portion of the EGFR, are the places of autophosphorylation upon binding with the EGF. Determining the level of expression of EGFR is necessary to establish a specific treatment with monoclonal antibodies (cetuximab). The EGFR is overexpressed in some tumors of the colon, esophagus, stomach, breast, CNS, bladder, lung, head and neck, uterine cervix, vulva, ovary and endometrium. EGFR is expressed in a high percentage of metastases of squamous cell carcinomas of different locations and therefore its expression has been considered as an indicator of poor prognosis in these tumors. The antibody detects both EGFR phosphorylated on Tyr1068 of the mature human isoform 1 (corresponding to Y1092 from the precursor form P00533-1/p170), and also unphosphorylated EGFR.

Composition: anti-human EGFR rabbit monoclonal antibody purified by protein affinity and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Immunogen: A synthetic phoshopeptide corresponding to residues Tyr1068 of human EGFR was used as immunogen.