دسته بندی: مونوکلونال

Name: Beta-catenin Antibody Clone EP35

Description and applications: Beta-Catenin is a key regulatory protein involved in cell adhesion and signal transduction through the Wnt pathway, and plays important roles in development, cellular proliferation, and differentiation. Mutations in the Beta-Catenin gene CTNNB1 leading to stabilization of Beta-Catenin in the cytoplasm and translocation to the nucleus have been implicated in various forms of tumor including familial adenomatous polyposis, fibromatosis, solitary fibrous tumors and endometrial carcinoma. A nuclear accumulation of Beta-Catenin in fibromatosis (desmoid tumor) in various locations including breast and mesentery is useful in the differentiation of this tumor from other fibroblast like lesions.

Composition: anti-human Beta-catenin rabbit monoclonal antibody purified and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Name: Rabbit Anti-Human Bcl-2 Monoclonal Antibody Clone EP36

Description and applications: The Bcl-2 family of proteins regulates apoptosis by controlling mitochondrial permeability and release of cytochrome c. Bcl-2 is an anti-apoptotic protein that resides in the outer mitochondrial wall and inhibits release of cytochrome c. Over-expression of Bcl-2 has been shown to promote cell survival by suppressing apoptosis. It has been documented that bcl-2 becomes deregulated in tumor cells as a result of translocation into the immunoglobulin heavy-chain locus and is therefore activated in B cell malignancies. Bcl-2 antibody is useful in differentiation of follicular lymphoma from reactive follicular proliferation (bcl-2 negative). In addition, bcl-2 expression has been shown to be correlated with disease prognosis in breast cancer, prostate cancer, ovarian cancer, endometrial cancer and colon cancer. The sensitivity of this antibody in diagnosis of follicular lymphoma Nucleolar dot-like staining is frequently seen with this antibody and it should not interfere with the staining interpretation.

Composition: Antibody to Bcl-2 is affinity purified and diluted in 10 mM Phosphate buffered saline (PBS), pH 7.2 containing 1% bovine serum albumin (BSA) and 0.09% sodium azide (NaN3).

Immunogen: A synthetic peptide corresponding to residues between BH3 and BH4 of human Bcl-2 protein

(Name: Mouse anti-human Cytokeratin 19  Monoclonal Antibody (Clone BA17

Description and Applications: Cytokeratin 19 is a member of type I acidic subfamily of intermediate filaments. It is expressed in variou different human tissues except in liver. Keratin 19 is not expressed in hepatocytes, therefore, antibody to keratin 19 is useful in the identification of liver metastasis. It is expressed by the great majority of breast carcinomas and used as a marker for sentinel lymph node evaluation both in frozen tissues and formalin fixed, paraffin embedded samples.

COMPOSITION: Anti-human Cytokeratin 19 mouse monoclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

IMMUNOGEN: Detergent soluble extract of human mammary epithelium.

.(Name: Mouse anti-human Androgen Receptor Monoclonal Antibody (Clone SP107

Description and applications: The expression of AR is reportedly inversely correlated with histologic grade i.e. well differentiated prostate tumors show higher expression than the poorly differentiated tumors. In prostate cancer, AR has been proposed as a marker of hormone-responsiveness. Composition: anti-human androgen receptor (AR) mouse monoclonal antibody purified from ascites. Prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Immunogen: A synthetic peptide from human AR.

(Name: Mouse anti-human Desmin Monoclonal Antibody (Clone D33

 Description and applications: Desmin is an intermediate filament protein of both smooth and striated muscles Antibody to desmin reacts with striated (skeletal and cardiac) as well as smooth muscle cells. In skeletal and cardiac muscles, the staining is confined to the Z-bands giving a characteristic striated appearance. Anti-desmin antibody is useful in identification of tumors of myogenic origin. It reacts with leiomyosarcomas (smooth muscle) as well as rhabdomyosarcomas (striated muscle). The antibody does not stain epithelial or myoepithelial cells, endometrial stromal tumors or ovarian cancer, lymphoid tissue, osteocytes, chondrocytes or mesothelial cells.

Composition: anti-human Desmin mouse monoclonal antibody purified from ascites. Prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Immunogen: Desmin from human muscle.

.(Name: Rabbit anti-human DOG-1 Monoclonal Antibody (Clone SP31

Description and aplications: Dog1 is a cell surface protein of unknown function selectively expressed in gastrointestinal stromal tumors (GIST). Among GIST cases with KIT mutations the Dog1 antibody identified 11% more cases than c-Kit antibody . Dog1 identifies vast majority of both c-Kit negative and PDGFRA mutated GIST cases that may still benefit from imatinib mesylate (Gleevac), an inhibitor of the kit tyrosin kinase. In addition, Dog1 immunoreactivity is seen in fewer cases of mesenchymal, epithelial tumours and melanomas when compared with c-Kit. The use of this highly sensitive and specific novel marker will increase the accuracy of GIST diagnosis.

 Composition: anti-human DOG1 rabbit monoclonal antibody purified from ascites. Prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Immunogen: Synthetic peptide of human DOG-1 protein.

.(Name: ERCC1 Antibody (Clone SP68

Description and applications: Nucleotide Excision Repair (NER) is a versatile mechanism capable of recognizing and eliminating damaged DNA, most commonly caused by UV radiations. ERCC1 is one of the three endonucleases that catalyzes this process.  The ERCC1 gene is located on the chromosomal region 19q13.3-q13.2 and encodes a protein of 297 amino acids and about 32.5 kDa molecular weight.  Although there are only few immunohistochemical studies with antibodies against ERCC1, it seems that tumors with extensive and intense immunohistochemical expression of this molecule have a worse prognosis and are resistant to platinum-based therapy. These tumors include non-small cell lung adenocarcinomas, gastrointestinal tract adenocarcinomas, breast, urinary bladder and adrenal cortex carcinomas, and advanced stage genital tract carcinomas.

Composition: anti-ERCC1 rabbit monoclonal antibody obtained from supernatant culture and prediluted in a tris buffered solution pH 7.4 containing 0.375mM sodium azide solution as bacteriostatic and bactericidal.

Immunogen: Recombinant human ERCC1 protein.

Name: Rabbit Anti-Human Estrogen Receptor (ER) Monoclonal Antibody clone SP1

Description and aplications: This rabbit monoclonal antibody (clone SP1) specifically reacts with the human alpha isoform of the estrogen receptors not having been tested in other species as mouse, rat and hamsters were it shows reactivity.This antibody is useful for immunohistochemical identification of estrogen receptor in normal and neoplastic cells estrogen dependent. However, the description of various types of benign and malignant neoplasms positive for estrogen receptors as meningiomas, lung and stomach adenocarcinoma, and above all in breast cancer, the amount of estrogen receptors expression in the tissue is an important parameter in predicting the prognosis of the disease and its response to endocrine therapy.

Presentation: ready-to-use

Composition: anti-ER rabbit monoclonal antibody obtained from supernatant culture and prediluted in a tris buffered solution pH 7.4 containing 0.375mM sodium azide solution as bacteriostatic and bactericidal.

Immunogen: Synthetic peptide derived from the C-terminus of human estrogen receptor alpha

Species reactivity: In vitro diagnostics in humans. Not tested in other species

.(Name: Factor XIIIa (FXIIIa) Antibody (Clone EP292

Description and applications: Factor XIII (plasma transglutaminase, fibrin stabilizing factor) is a glycoprotein that circulates in blood as a tetramer, consisting of two A and two B subunits. Subunit A of factor XIII is an un-glycosylated 730-residue peptide with a molecular mass of 83 kD. It is the last enzyme generated in the blood coagulation cascade and is the zymogen for fibrinoligase, a transglutaminase that forms intramolecular gamma-glutamyl-epsilon-lysine crosslinking between fibrin molecules and thus stabilizes blood clots.

Factor XIII A is present in plasma, platelets, and monocytes, as well as macrophages and bone marrow precursors of these cell types and selective expression in subsets of stromal inflammatory cells. Factor XIII A is a marker for alternatively activated macrophages, while absence of Factor XIII A in monocyte-derived

macrophages is an indicator of their activated state. In addition, Factor XIII A is useful in distinguishing malignant fibrous histiocytoma (positive) from soft tissue tumor (negative).

Composition: anti-human FXIIIa rabbit monoclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Immunogen: A synthetic peptide corresponding to residues of human Factor XIII A protein.

(Name: Rabbit anti-human FSH (Follicle Stimulating Hormone) Monoclonal Antibody (Clone EP257

Description and applications: The pituitary glycoprotein hormone family includes follicle-stimulating  hormone, luteinizing hormone, chorionic gonadotropin, and thyroid-stimulating hormone. All of these glycoproteins consist of an identical alpha subunit and a hormone-specific beta subunit. FSH beta is the beta subunit of folliclestimulating hormone. In conjunction with luteinizing hormone, follicle-stimulating hormone induces egg and sperm production. The FSH antibody specifically labels Gonadotrophs in pituitatry. It may be useful in the classification of pituitary tumors.

Composition: Anti-human FSH (Follicle Stimulating Hormone) rabbit monoclonal antibody purified from . serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Immunogen: A synthetic peptide corresponding to residues of human FSH (beta subunit) protein.

.(Name: Galectin 3  Antibody (Clone B2C10

Description and applications: The antibody recognizes a 30 kDa protein member of the family of the beta-galactosidase-binding lctin involved in cell growth, adhesion, inflammation and apoptosis processes. Galectin-3 is expressed in normal inflammatory cells (granulocytes) in vascular endothelium and in primary and metastatic malignancies of the colon, liver, breast, tongue and thyroid. This antibody is useful for differentiating between benign (adenomas) and thyroid malignancies. It may also be useful for the recognition of minimally invasive thyroid lesions. The combined use of Galectin-3 and HBME-1 may be helpful in the differential diagnosis between adenomas and oncocytic thyroid carcinomas. The sensitivity of galectin-3 to detect oncocytic carcinomas and the oncocytic variant of papillary carcinoma is 95%. The medullary carcinomas and normal thyroid follicles do not express galectin-3.

Composition: anti-Galectin 3 mouse monoclonal antibody obtained from supernatant culture and prediluted in a tris buffered solution pH 7.4 containing 0.375mM sodium azide solution as bacteriostatic and bactericidal.

Immunogen: Recombinant protein of the full length od the human Galectin-3 protein.

.(Name: GATA3 Antibody (Clone L50-823

Description and applications: GATA3 (GATA binding protein 3 to DNA sequence [A/T]GATA[A/G]) is a zinc finger transcription factor and plays an important role in promoting and directing cell proliferation, development, and differentiation in many tissues and cell types. GATA3 expression is primarily seen in breast carcinoma and urothelial carcinoma and only rarely found in tumors from other organs, such as endometrial endometrioid adenocarcinoma or yolk sac tumors. In the lymphoid system GATA-3 gene expression promotes receptor T cells and induces the activation of T helper type 2 cells. GATA3 is expressed in all breast lobular carcinomas and 91% of invasive ductal carcinomas (grade I, 100%; grade II, 89% and grade III, 86%1-2). GATA3 expression seems to be lower in luminal B subtype breast carcinoma. It has also been reported that GATA3 expression is correlated with the status of ER, PR and Her2 in breast carcinoma. GATA3 expression is found in urothelial carcinoma, especially in invasive and high grade tumors. Therefore, anti-GATA3 can be used in a panel of antibodies for diagnosis of unknown primary carcinoma, when carcinomas of the breast or bladder are a possibility. Isolated studies have demonstrated the usefulness of GATA-3, together with T-bet marker to differentiate between lymphocytic colitis and celiac disease. In germ cell tumors glandular areas of endodermal and trophoblastic differentiation might be stained. GATA 3 is also positive in many tissues such as parathyroid or salivary gland tumors; mucinous and non-mucinous pancreatic adenocarcinomas, squamous cell carcinomas, basal cell carcinomas, chromophobe carcinomas of the kidney, mesotheliomas, poorly differentiated papillary or mucinous adenocarcinomas of the lung, carcinomas of the stomach, colon, prostate, endometrium, thyroid, serous and Brenner benign and borderline ovarian tumors.

Composition: anti-human GATA3 mouse monoclonal antibody purified from ascites. Prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.