(Name:Mouse Anti-p27 Monoclonal Antibody (Clone DCS-72.F6
Description and aplications: This antibody recognizes a protein of 27 kDa identified as p27kip1, a mitotic inhibitor cell cycle regulator. The epitope extends between amino acids 83 to 197 of p27. This antibody is highly specific and does not cross react with other mitotic inhibitors. The p27 functions as a negative regulator of G1 progression and has been proposed as a possible mediator of cell G1 arrest induced by TGF-Beta. Is structurally related with Kip1 Cip1 / WAF1, with a similar sequence of 60 amino acids at the N-terminal region. In vitro, p27Kip1 strongly binds to cyclin D-Cdk4, cyclin E-Cdk2 and cyclin A-Cdk2 and inhibits cdk activity. In normal cells the level of Kip1 activity gradually decreases as the cells reach the stage S. The addition of TGF-b in early G1 prevents this decrease of activity, apparently by preventing Kip1 sequestration that occurs in cells that have not been treated with this factor. The treatment with TGF-b also reduces the levels of Cdk4. Kip1 preferentially binds to cyclin D-Cdk4, but the lower levels of Cdk4 in cells treated by TGF-b, presumably allow for some of the Kip1 to bind to the cyclin E-Cdk2 and cyclin A-Cdk2.
Composition: anti-p27 mouse monoclonal antibody obtained from supernatant culture and prediluted in atris buffered solution pH 7.4 containing 0.375mM sodium azide solution as bacteriostatic and bactericidal.
.Immunogen: mouse p27 recombinant protein Ig
