immunohistochemistry

Name: INI-1 (BAF47/SNF5) Antibody (Clone 25/BAF47)

Description and applications: SWI/SNF complexes facilitate gene activation and transcription factor binding by altering repressive chromatin structures in an ATP-dependent manner. In mammals, SWI/SNF complexes are present in mutliple forms that include 9-12 BRG1-associated factors (BAFs) per complex. These BAF proteins range in molecular weight from 47 to 250 kDa. BAF47/SNF5 (SMARCB1/Ini1) complexes with BRG1- and BRM-containing SWI/SNF complexes. BAF47/SNF5 mRNA is widely expressed in cell lines and tissues, and BAF47/SNF5 gene is mutated in many human tumors. The latter is indicative of a tumor suppressor role for BAF47/SNF5. In addition, mice deficient for BAF47 die early in embryogenesis, while BAF47/SNF5 heterozygous mice display a variety of tumors in the soft tissues of the head and neck. BAF47/SNF5 also binds the HIV-1 integrase and stimulates integrase-mediated DNA joining activity. Thus, BAF47/SNF5 is a component of SWI/SNF complexes that may be critical for normal development and tumor suppression, but may also be a protein utilized for viral DNA integration into host DNA. Expression in normal tissues is ubiquitous. In tumor tissue loss of protein expression SMARCB1 / INI1 gene was first observed in all malignant rhabdoid tumors of childhood of different location (soft tissue, kidney and CNS) and in medullary carcinomas of the kidney. Also loss of expression is observed in 50% of synovial sarcomas, some myxoid chondrosarcoma and myoepithelial carcinomas, almost all epithelioid sarcoma (proximal and conventional variants) and 50% of malignant tumors of peripheral nerves sheat, all presenting rhabdoid morphology. Loss of immunohistochemistry expression is also observed in cases of poorly differentiated chordoma. The antibody is useful in differentiating rhabdoid tumors of other tumors with similar morphology as desmoplastic round cell tumor, rhabdomyosarcoma and Wilms tumor do not show loss of expression of INI1. The antibody also is useful in confirming the diagnosis of medullary carcinoma and discard possible renal pelvis urothelial carcinoma or other carcinomas of the kidney showing expression for INI1. Maintained staining in most carcinomas, epithelioid angiosarcoma, mesotheliomas, melanomas and other tumors or lesions reagents (granulomas) of epithelioid morphology make this useful antibody in the diagnosis of epithelioid sarcoma.

Composition: anti-human INI-1 mouse monoclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Immunogen: mouse BAF47 aminoacids 257-359.

Name: Muscle Specific Actin,Antibody (Clone HHF35)

Description and applications: Actin is a major component of the cytoskeleton. This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta and gamma in order of increasing isoelectric point. Anti- Muscle-Specific Actin recognizes alpha and gamma isotypes of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-musclederived tissue such as carcinomas, melanomas, and lymphomas are negative. This antibody is useful in the identification of rhabdoid cellular elements.

Composition: anti-human Muscle Specific Actin mouse monoclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Immunogen: SDS extract of human myocardium

Name: CD10 Antibody (Clone 56C6)

Description and aplications: CD10, also known as Common Acute Lymphocytic Leukemia Antigen (CALLA), is a cell surface enzyme with neutral metalloendopeptidase activity which inactivates a variety of biologically active peptides. CD10 is expressed on the cells of lymphoblastic, Burkitt’s, and follicular germinal center lymphomas, and on cells from patients with chronic myelocytic leukemia (CML). It is also expressed on the surface of normal early lymphoid progenitor cells, immature B cells within adult bone marrow and germinal center B cells within lymphoid tissue. CD10 is also present on breast myoepithelial cells, bile canaliculi, fibroblasts, with especially high expression on the brush border of kidney and gut epithelial cells.

Composition: anti-human CD10 mouse monoclonal antibody purified from ascites fluid by Protein A chromatography. Prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Name: CD103  Antibody (Clone EP206)

Description and applications: CD103, also known as integrin alpha E (ITGAE), is an integrin protein that in humans is encoded by the ITGAE gene. It binds integrin beta 7 to form the complete heterodimeric molecular αEβ7 that binds to an extracellular matrix component and cellular counter receptor. They mediate cell adhesion, migration and signaling and are important for T lymphocyte localization. CD103 is expressed on intraepithelial lymphocytes in mucosal areas, including lung and GI tract. In malignancies, CD103 is present on all enteropathy-type T-cell lymphomas. Additionally, CD103 has been a useful marker for hairy cell leukemia.

Composition: anti-human CD130 rabbit monoclonal antibody purified from ascites. Prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Name: CD117/c-kit Antibody (Clone EP10)

Description and applications: This antibody reacts with human oncoprotein c-kit (CD117). The proto-oncogene c-kit encodes a transmembrane receptor with tyrosine kinase activity, c-kit (CD117), which is closely related to the family of platelet derived growth factor receptors. c-KIT is involved in hematopoiesis, gametogenesis and melanogenesis. This antigen is expressed in the normal breast epithelium, melanocytes, mast cells and glia. This antibody is recommended to identify oncoprotein expression c-kit in a variety of normal and neoplastic tissues, including gastrointestinal stromal tumors (GIST). It is also expressed in testicular seminoma, small cell carcinomas of the lung, breast carcinomas, glioblastomas, melanomas, and chronicmyeloid leukemias or acute myeloid leukemias in myeloid blast crisis.

Composition: anti-human CD117/c-kit rabbit monoclonal antibody purified from ascites. Prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Name: Rabbit anti-human CD13 Monoclonal Antibody

Composition: anti-human CD13 rabbit monoclonal antibody purified from ascites fluid by chromatography. Prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide

Intended use: Immunohistochemistry (IHC) on paraffin embedded tissues. Not tested on frozen tissues or Western-Blotting

Immunogen: A synthetic peptide corresponding to residues in human CD13 protein

Visualization: Cell membrane

Name:CD138 Antibody clone EP201

Description and applications: CD138, also known as Syndecan-1, is a member of the transmembrane heparan sulfate proteoglycan family, acts as an extracellular matrix receptor and is involved in many cellular functions, including cell-cell adhesion and cell-matrix adhesion. CD138 expression is found in both hematopoietic and non-hematopoietic cells. In the hematopoietic system, CD138 labels plasma cells. It is an excellent marker for plasmacytic differentiation within the spectrum of hematologic malignancy. It´s a very usefull tool in the diagnostic of chronic endometritis associated with infertility. Among nonhematolymphoid cells, CD138 reactivity is observed inmany types of epithelial cells and stoma cells in both normal and tumor tissues. These staining should
be remembered and not interpreted as unspecific.

Composition:Anti-human CD138 rabbit monoclonal antibody purified from serum and prepared in 10mM PBS, pH
7.4, with 0.2% BSA and 0.09% sodium azide

Intended use: Immunohistochemistry (IHC) on paraffin embedded tissues. Not tested on frozen tissues or Western-Blotting

Name: CD14 Antibody (Clone EP128)

Description and applications:CD14 is a 55-kDa protein found as a glycosylphosphatidylinositol (GPI)- anchored protein on the surface of monocytes, macrophages, and polymorphonuclear leukocytes, and as a soluble protein in the blood. Its main function is to serve as a receptor for lipopolysaccharide (LPS). Besides its role in endotoxin signaling, it has been proposed that CD14 is involved in the transportation of other lipids, cell-cell interactions during different immune responses, and recognition of apoptotic cells.
CD14 is highly expressed on the surface of monocytes/macrophages and strongly up-regulated during the differentiation of monocytic precursor cells into mature monocytes. Therefore, CD14 has been commonly used as a differentiation marker for monocytes/macrophages. An antibody to CD14 also labels Langerhans cells and dendritic cells.

Composition: Anti-human CD14 rabbit monoclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.